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Brug RNA-sekventering til Detect Novel Splice Varianter Relateret til Drug Resistance i<em> In vitro</em> Kræft Modeller
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
DOI:

09:58 min

December 09, 2016

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Chapters

  • 00:05Title
  • 01:09Characterization of Drug Resistance Profiles through Cytotoxicity Assays
  • 05:01RNA Isolation and Library Preparation for RNA-sequencing
  • 05:55Validation of the Results by RT-PCR and qRT-PCR Assays
  • 07:21Results: Detection of Differential Splicing Events in Chemotherapy-resistant Cell Lines Compared to Parental Wild-type by Using RNA-sequencing
  • 09:07Conclusion

Summary

Automatic Translation

Her beskriver vi en protokol til formål at undersøge effekten af ​​afvigende splejsning på resistens i solide tumorer og blodkræftsygdomme. Til dette mål, vi analyserede transkriptom profiler af forældrenes og resistente in vitro modeller gennem RNA-seq og etablerede et QRT-PCR-metode til at validere kandidatgener.

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