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Cancer Research
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利用RNA测序来检测新剪接变异体在相关耐药<em>体外</em>癌症模型
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Cancer Research
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JoVE Journal
Cancer Research
Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in
In Vitro
Cancer Models
Please note that all translations are automatically generated.
Click here for the English version.
利用RNA测序来检测新剪接变异体在相关耐药<em>体外</em>癌症模型
DOI:
10.3791/54714-v
•
09:58 min
•
December 09, 2016
•
Rocco Sciarrillo
2,3
,
Anna Wojtuszkiewicz
,
Irsan E. Kooi
,
Valentina E. Gómez
,
Ugo Boggi
,
Gerrit Jansen
,
Gert-Jan Kaspers
7
,
Jacqueline Cloos*
1
,
Elisa Giovannetti*
3,8,9
1
Department of Pediatric Oncology/Hematology
,
VU University Medical Center
,
2
Department of Hematology
,
VU University Medical Center
,
3
Department of Medical Oncology
,
VU University Medical Center
,
4
Department of Clinical Genetics
,
VU University Medical Center
,
5
Division of General and Transplant Surgery, Azienda Ospedaliera Universitaria Pisana
,
Universita’ di Pisa
,
6
Amsterdam Immunology and Rheumatology Center
,
VU University Medical Center
,
7
Princess Máxima Center for Pediatric Oncology
,
8
Cancer Pharmacology Lab, AIRC Start-Up Unit
,
University of Pisa
,
9
Institute of Nanoscience and Nanotechnology
,
CNR-Nano
Chapters
00:05
Title
01:09
Characterization of Drug Resistance Profiles through Cytotoxicity Assays
05:01
RNA Isolation and Library Preparation for RNA-sequencing
05:55
Validation of the Results by RT-PCR and qRT-PCR Assays
07:21
Results: Detection of Differential Splicing Events in Chemotherapy-resistant Cell Lines Compared to Parental Wild-type by Using RNA-sequencing
09:07
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里,我们描述旨在调查异常剪接的耐药性在实体瘤和血液恶性肿瘤的影响的协议。为了这个目标,我们通过RNA测序分析,家长
和体外
抗车型的转录配置文件,并建立了定量RT-PCR为基础的方法来验证候选基因。
Tags
RNA-sequencing
Splice Variants
Drug Resistance
In Vitro Cancer Models
Molecular Pharmacology
Chemotherapy Resistance
Transcriptomic Analysis
Cytotoxicity Screening
MTT Assay
Leukemic Cells
Dexamethasone
Formazan Crystals
Microplate Reader
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