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Neuroscience
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利用3D打印技术来合并MRI与组织学:一个大脑切片协议
JoVE Journal
Neuroscience
Author Produced
This content is Free Access.
JoVE Journal
Neuroscience
Utilizing 3D Printing Technology to Merge MRI with Histology: A Protocol for Brain Sectioning
Please note that all translations are automatically generated.
Click here for the English version.
利用3D打印技术来合并MRI与组织学:一个大脑切片协议
DOI:
10.3791/54780-v
•
15:53 min
•
December 06, 2016
•
Nicholas J Luciano
,
Pascal Sati
,
Govind Nair
,
Joseph R Guy
,
Seung-Kwon Ha
,
Martina Absinta
,
Wen-Yang Chiang
,
Emily C Leibovitch
,
Steven Jacobson
,
Afonso C Silva
,
Daniel S. Reich
1
Translational Neuroradiology Section
,
National Institute of Neurological Disorders and Stroke
,
2
Cerebral Microcirculation Section
,
National Institute of Neurological Disorders and Stroke
,
3
Viral Immunology Section
,
National Institute of Neurological Disorders and Stroke
Chapters
00:00
Title
00:36
MRI Preparation and Acquisition
01:53
Extracting Brain Surface
04:06
Selecting Slice Location
05:05
Creating MRI Blade Map
05:46
Imaging Brain and Blade Gap Surfaces
07:27
Editing Brain Contours
07:50
Creating the Brain Slicer Box
11:25
Printing the Brain Slicer Box
12:16
Cutting the Brain
13:16
Representative Results
15:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议的总体目标是通过创建定制3D印刷脑持有者和切片机盒的精确对准与组织切片的磁共振成像(MRI)图像的卷。
Tags
3D Printing
MRI
Histology
Brain Sectioning
Marmoset
Formalin
Fomblin
T2-weighted Imaging
Image Processing
Noise Reduction
Segmentation
Binary Mask
Morphological Operations
Surface Extraction
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