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La fabricación de un UV-Vis y espectroscopia Raman Plataforma de Inmunoensayo
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
DOI:

09:02 min

November 10, 2016

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Chapters

  • 00:05Title
  • 01:06Preparation of PEGylated Antibody (PEG-Ab) Solution
  • 02:25Prepare UV-Vis/Raman Probes
  • 04:57Immunoassay Plate Preparation
  • 06:08Functionalizing the Immunoassay Plate
  • 07:18Test Assay Sensitivity by UV-Vis and Raman Spectroscopy
  • 07:58Results: Determining the Sensitivity of UV-Vis and Raman Spectroscopy
  • 08:39Conclusion

Summary

Automatic Translation

Nanoparticle-based optical probes have been designed as a vehicle for detecting antigens using Raman and UV-Vis spectroscopy. Here we describe a protocol for preparing such probes for a UV-Vis/Raman spectroscopy immunoassay in such a way to incorporate future multiplexing capabilities.

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