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Journal / Biochemistry / Un protocolo optimizado para el ensayo de cambio…
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Biochemistry

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JoVE Journal Biochemistry
Un protocolo optimizado para el ensayo de cambio de movilidad electroforética utilizando oligonucleótidos marcados con colorante fluorescente infrarrojo
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Un protocolo optimizado para el ensayo de cambio de movilidad electroforética utilizando oligonucleótidos marcados con colorante fluorescente infrarrojo

Article DOI: 10.3791/54863-v • 09:58 min • November 29th, 2016
November 29th, 2016 •
1, 1, 1
1Department of Biological Sciences, University of Illinois at Chicago

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Se describe aquí un protocolo optimizado de ensayos fluorescentes cambio de movilidad electroforética (FEMSA) utilizando purificados Sox-2 proteínas, junto con sondas fluorescentes infrarrojos marcado tinte de ADN como un estudio de caso para hacer frente a una importante cuestión biológica.

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Bioquímica No. 117 fluorescente EMSA tinte fluorescente en el infrarrojo sistema de imágenes de infrarrojos, inhibidores de la HMG-box factor de transcripción identidades neuronales postmitotic
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