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Genetics
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使用了大气颗粒物引起的染色体畸变分析<em>体外</em>系统
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JoVE Journal
Genetics
Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an
In Vitro
System
Please note that all translations are automatically generated.
Click here for the English version.
使用了大气颗粒物引起的染色体畸变分析<em>体外</em>系统
DOI:
10.3791/54969-v
•
08:48 min
•
December 21, 2016
•
Isabelle R. Miousse
,
Igor Koturbash
,
Marie-Cécile Chalbot
,
Martin Hauer-Jensen
,
Ilias Kavouras
,
Rupak Pathak
1
Department of Occupational and Environmental Health
,
University of Arkansas for Medical Sciences
,
2
Department of Environmental Health Sciences
,
University of Alabama at Birmingham
,
3
Division of Radiation Health
,
University of Arkansas for Medical Sciences
Chapters
00:05
Title
00:35
Particle Exposure
02:12
Cell Treatment with Particulate Matter
03:21
Cell Harvest, Hypotonic Treatment, and Fixation
05:35
Slide Cleaning and Metaphase Spread Preparation
07:12
Results: Chromosomal Aberrations from Particulate Matter on Metaphase Spreads
08:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议与周围空气的微粒物质处理后
描述
了在RAW264.7小鼠巨噬细胞
体外
量化和染色体畸变的表征技术。
Tags
Chromosomal Aberrations
Particulate Matter
In Vitro
Air Pollution
Cell Culture
Genotoxicity
Metaphase Arrest
Trypsin
Cell Suspension
Cell Density
Cell Incubation
PBS
Colcemid
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