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Analyse van de Ambient Particulate Matter-geïnduceerde chromosomale afwijkingen met behulp van een<em> In Vitro</em> Systeem
JoVE Journal
Genetics
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JoVE Journal Genetics
Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
DOI:

08:48 min

December 21, 2016

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Chapters

  • 00:05Title
  • 00:35Particle Exposure
  • 02:12Cell Treatment with Particulate Matter
  • 03:21Cell Harvest, Hypotonic Treatment, and Fixation
  • 05:35Slide Cleaning and Metaphase Spread Preparation
  • 07:12Results: Chromosomal Aberrations from Particulate Matter on Metaphase Spreads
  • 08:08Conclusion

Summary

Automatic Translation

Dit protocol beschrijft technieken voor de kwantificatie en karakterisatie van chromosomale afwijkingen in vitro in RAW264.7 muis macrofagen na behandeling met de lucht zwevende deeltjes.

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