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Protocol to Create Chronic Wounds in Diabetic Mice
JoVE Journal
Medicine
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JoVE Journal Medicine
Protocol to Create Chronic Wounds in Diabetic Mice
DOI:

06:55 min

September 25, 2019

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Chapters

  • 00:04Title
  • 00:26Shaving and Application of Depilatory Lotion
  • 03:09The Wound Model
  • 05:47Results: Wound Progression to Closure or Chronicity
  • 06:29Conclusion

Summary

Automatic Translation

Chronic wounds are developed from acute wounds on a diabetic mouse model by inducing high levels of oxidative stress after a full-thickness cutaneous wound. The wound is treated with inhibitors for catalase and glutathione peroxidase, resulting in impaired healing and biofilm development by bacteria present in the skin microbiome.

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