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Journal / Immunology and Infection / Bir TGF-β-içeren Protokolü kullanılarak naif CD4…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Bir TGF-β-içeren Protokolü kullanılarak naif CD4 + T hücrelerinden insan CD4 + FoxP3 + Bağlı regülatör T hücreleri (iTregs) in vitro ayrımında
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Journal (Immunology and Infection)
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Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
Journal (Immunology and Infection)
Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
 
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Bir TGF-β-içeren Protokolü kullanılarak naif CD4 + T hücrelerinden insan CD4 + FoxP3 + Bağlı regülatör T hücreleri (iTregs) in vitro ayrımında

Article DOI: 10.3791/55015-v • 08:20 min • December 30th, 2016
December 30th, 2016 •
1, *1, *1, 1
1Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory
* These authors contributed equally

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Bu protokol, in vitro olarak naif CD4 + T hücrelerinden üretilebilir üretimi ve insan kaynaklı düzenleyici T hücrelerinin (iTregs) fenotipleme tarif eder. FoxP3 indüksiyonu için farklı protokoller ilgili protokolleri ile elde edilen spesifik iTreg fenotipleri çalışması için izin verir.

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Immunology Sayı 118 düzenleyici T hücreleri regülatör CD4 + T hücreleri manyetik hücre izolasyonu, sitokinler FoxP3 TGF-β IL-2 hücre içi sitometrisi QRT-PCR Akış
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