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Naif CD4 adenoviral İletimi Treg Farklılaşma Eğitim Hücreler T
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
DOI:

15:33 min

August 13, 2013

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Chapters

  • 00:05Title
  • 02:03Cloning of a Gene of Interest into an Entry Vector
  • 03:16Generation of the Primary Virus Lysate
  • 05:47Virus Amplification
  • 07:34T Cell Infection
  • 09:01T Cell Activation and Polarization
  • 10:01T Cell Fixation Staining for Flow Cytometry
  • 11:51Infection Does Not Interfere with T Cell Activation
  • 14:42Conclusion

Summary

Automatic Translation

Naif CD4 Coxsackie adenovirüs reseptör transgenik ekspresyonu ile T-hücreleri içine adenoviral gen transferi düzenleyici T hücre farklılaşmasının moleküler analiz sağlar<em> In vitro</em>.

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