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应用先进的背皮窗室对转基因荧光小鼠肿瘤相关血管的活体显微镜观察
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice
Please note that all translations are automatically generated.
Click here for the English version.
应用先进的背皮窗室对转基因荧光小鼠肿瘤相关血管的活体显微镜观察
DOI:
10.3791/55115-v
•
08:52 min
•
January 19, 2018
•
Ann L.B. Seynhaeve
,
Timo L.M. ten Hagen
1
Laboratory Experimental Surgical Oncology, Section Surgical Oncology, Department of Surgery
,
Erasmus MC
Chapters
00:05
Title
00:47
Dorsal Tissue Implantation Under a Window Chamber
03:55
Intravital Imaging
06:37
Results: Intravital Tumor Monitoring
08:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了表达细胞特异荧光标记的转基因小鼠的活体成像。活体成像提供了一种非侵入性的方法, 用于高分辨率观察活体动物使用植入窗口, 通过它的微观可视化过程是可能的。这种方法对于研究纵向过程尤其有用。
Tags
Intravital Microscopy
Tumor Vasculature
Dorsal Skinfold Window Chamber
Transgenic Fluorescent Mice
Tumor Implantation
Syngeneic Tumor
Xenograft
Surgical Procedure
Aseptic Conditions
Hair Removal
Skin Preparation
Frame Installation
Glass Filler
Cover Glass
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