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Gelijktijdige meting van mitochondriale calcium en mitochondriale membraan potentieel in levende cellen door fluorescentie microscopie
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Gelijktijdige meting van mitochondriale calcium en mitochondriale membraan potentieel in levende cellen door fluorescentie microscopie

Article DOI: 10.3791/55166-v • 08:43 min • January 24th, 2017
January 24th, 2017 •
1,2, 1, 3
1Centre for Genetic Diseases, Hudson Institute of Medical Research, 2The Department of Molecular and Translational Sciences, Monash University, 3Department of Physiology, University College London

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Mitochondriën kan de elektrochemische potentiaal over hun binnenste membraan (Δ Ψm) te gebruiken om calcium sekwestreren (Ca 2+), waardoor ze vorm te geven cytosolische Ca 2+ het signaleren binnen de cel. We beschrijven een werkwijze voor het gelijktijdig meten van mitochondriën Ca2 + opname en ΔΨ m in levende cellen met fluorescerende kleurstoffen en confocale microscopie.

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Cellular Biology Mitochondriën membraanpotentiaal calcium fluorescerende kleuring levende cellen confocale microscopie
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