/
/
Analizando Synaptic Modulación de<em> Drosophila melanogaster</em> Los fotorreceptores después de la exposición prolongada a la luz
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Neuroscience of Disease, Center for Transdisciplinary Research,Niigata University, 2Brain Research Institute,Niigata University, 3Image and Data Analysis Facility,German Center for Neurodegenerative Diseases (DZNE), 4Graduate School of Life Science and Technology,Tokyo Institute of Technology (Titech), 5Dendrite Differentiation,German Center for Neurodegenerative Diseases (DZNE)
Chapters
- 00:05Title
- 00:47Treatment of Flies and Imaging Brains
- 02:54Image Analysis: Generating the Spots, Surface Objects, Start-points, and End-points
- 08:04Image Analysis: Calculating the Number of Spots, Distribution of Spot Frequency and Enrichment Level of Brp-GFP
- 09:48Results: Brp-GFP Expression in R8 Synapses of the Medulla
- 11:02Conclusion
Aquí mostramos cómo cuantificar el número y la distribución espacial de las zonas activas sinápticas en los fotorreceptores de Drosophila melanogaster, resaltada con un marcador molecular codificada genéticamente, y su modulación después de la exposición prolongada a la luz.
