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Neuroscience
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分析突触调制<em>果蝇</em>曝光时间延长后,光光感受器
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Analyzing Synaptic Modulation of
Drosophila melanogaster
Photoreceptors after Exposure to Prolonged Light
Please note that all translations are automatically generated.
Click here for the English version.
分析突触调制<em>果蝇</em>曝光时间延长后,光光感受器
DOI:
10.3791/55176-v
•
11:36 min
•
February 10, 2017
•
Atsushi Sugie
2,5
,
Christoph Möhl
,
Satoko Hakeda-Suzuki
,
Hideaki Matsui
2
,
Takashi Suzuki*
4
,
Gaia Tavosanis*
5
1
Department of Neuroscience of Disease, Center for Transdisciplinary Research
,
Niigata University
,
2
Brain Research Institute
,
Niigata University
,
3
Image and Data Analysis Facility
,
German Center for Neurodegenerative Diseases (DZNE)
,
4
Graduate School of Life Science and Technology
,
Tokyo Institute of Technology (Titech)
,
5
Dendrite Differentiation
,
German Center for Neurodegenerative Diseases (DZNE)
Chapters
00:05
Title
00:47
Treatment of Flies and Imaging Brains
02:54
Image Analysis: Generating the Spots, Surface Objects, Start-points, and End-points
08:04
Image Analysis: Calculating the Number of Spots, Distribution of Spot Frequency and Enrichment Level of Brp-GFP
09:48
Results: Brp-GFP Expression in R8 Synapses of the Medulla
11:02
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们将展示如何量化的数量和
果蝇
光感受器突触活动区域的空间布局,突出了遗传编码的分子标记,并长时间暴露于光后的调制。
Tags
Synaptic Modulation
Drosophila Melanogaster
Photoreceptors
Prolonged Light Exposure
Synaptic Plasticity
Synaptic Dynamics
Neuron Activity
Synaptic Analysis
Brp Expression
R7 And R8 Photoreceptor Axons
Immunolabeling
3D Imaging
Image Analysis
Spot Detection
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