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Analysere Synaptic Modulation av<em> Drosophila melanogaster</em> Fotoreseptorer etter eksponering for Langvarig lys
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light
DOI:

11:36 min

February 10, 2017

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Chapters

  • 00:05Title
  • 00:47Treatment of Flies and Imaging Brains
  • 02:54Image Analysis: Generating the Spots, Surface Objects, Start-points, and End-points
  • 08:04Image Analysis: Calculating the Number of Spots, Distribution of Spot Frequency and Enrichment Level of Brp-GFP
  • 09:48Results: Brp-GFP Expression in R8 Synapses of the Medulla
  • 11:02Conclusion

Summary

Automatic Translation

Her viser vi hvordan du kan kvantifisere antallet og romlig fordeling av synaptiske aktive soner i Drosophila melanogaster fotoreseptorer, markert med en genetisk kodet molekylær markør, og deres modulering etter langvarig eksponering for lys.

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