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Rapid Deletion Produksjon i Fungi via<em> Agrobacterium</em> Mediated Transformation of OSCAR Deletion Constructs
JoVE Journal
Genetics
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JoVE Journal Genetics
Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs
DOI:

11:24 min

June 12, 2017

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Chapters

  • 00:05Title
  • 01:02Primer Design for PCR Amplification of Gene Flanks
  • 02:49Production of OSCAR Constructs
  • 05:48Agrobacterium tumefaciens Mediated Transformation (ATMT) of Fungi
  • 07:45Deletion Mutant Identification by PCR
  • 08:53Results: The OSCAR Method Generates a Gene Deletion Plasmid for Fungi in a Single Reaction
  • 10:23Conclusion

Summary

Automatic Translation

Gene-deletjonsmutanter generert ved homolog rekombination er gullstandarden for genfunksjonsstudier. OSCAR (One Step Construction av Agrobacterium-Recombination-ready-plasmids) metode for rask generering av deletjonskonstruksjoner er beskrevet. Agrobacterium- mediert sopptransformasjon følger. Endelig presenteres en PCR-basert bekreftelsesmetode for genutslettelser i sopptransformanter.

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