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Journal
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Immunology and Infection
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细胞内染色和流式细胞仪识别鼠主动脉,肾脏和淋巴结内淋巴细胞亚群的高血压模型
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension
Please note that all translations are automatically generated.
Click here for the English version.
细胞内染色和流式细胞仪识别鼠主动脉,肾脏和淋巴结内淋巴细胞亚群的高血压模型
DOI:
10.3791/55266-v
•
09:20 min
•
January 28, 2017
•
Fanny Laroumanie
,
Bethany L. Dale
,
Mohamed A. Saleh
,
Meena S. Madhur
1
Department of Medicine, Division of Clinical Pharmacology
,
Vanderbilt University Medical Center
,
2
Department of Molecular Physiology and Biophysics
,
Vanderbilt University
,
3
Department of Pharmacology and Toxicology, Faculty of Pharmacy
,
Mansoura University
Chapters
00:05
Title
00:46
Kidney, Aorta, and Lymph Node Isolation and Single Cell Suspension Generation
04:29
Ex Vivo
Stimulation and Intracellular Cytokine Detection
07:07
Results: Representative Analyses of Kidney, Aorta, and Aortic Lymph Node T Cell Activation
08:23
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文提供了详细的方法来识别和量化细胞内染色鼠肾,主动脉和淋巴结中存在功能性T淋巴细胞亚群和流式细胞仪。被选中的血管紧张素II诱导高血压模型来解释,一步一步的程序和流式细胞仪的基本原则和细胞内染色。
Tags
Intracellular Staining
Flow Cytometry
Lymphocyte Subsets
Murine Aorta
Kidney
Lymph Nodes
Hypertension
Angiotensin II
Single-cell Suspension
Immune Cells
Cardiovascular Disease
Perfusion
Dissection
Density Gradient Centrifugation
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