Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Rekonstituering af nucleosomer med differentielt…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Rekonstituering af nucleosomer med differentielt isotopmærkede Sister Histoner
SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry
Journal (Biochemistry)
SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry
Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
Journal (Biochemistry)
Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
 
Click here for the English version

Rekonstituering af nucleosomer med differentielt isotopmærkede Sister Histoner

Article DOI: 10.3791/55349-v • 09:26 min • March 26th, 2017
March 26th, 2017 •
1
1Department of Structural Biology, Leibniz Institute of Molecular Pharmacology (FMP-Berlin)

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Denne protokol beskriver rekonstituering af nukleosomer indeholder differentielt isotopmærkede søster histoner. Samtidig kan asymmetrisk posttranslationelt modificerede nukleosomer genereres efter anvendelse af en premodified histon kopi. Disse præparater kan let anvendes til at studere modifikation krydstale mekanismer, samtidigt på begge søster histoner, ved hjælp af høj opløsning NMR-spektroskopi.

Tags

Biochemistry histoner post-translationelle modifikationer nukleosomer NMR-spektroskopi epigenetik kromatin
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter