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对于电生理和钙成像急性神经组织的长时间培养
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JoVE Journal Neuroscience
Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging
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对于电生理和钙成像急性神经组织的长时间培养

DOI:
10.3791/55396-v

•

10:30 min

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February 15, 2017

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, , , , , , , ,
1The MARCS Institute,Western Sydney University, 2School of Medicine,Western Sydney University

Chapters

  • 00:05Title
  • 01:11Brain Slice and Retinal Wholemount Preparation
  • 04:02Calcium Dye Loading
  • 05:38Electrophysiological Recordings and Imaging
  • 07:28Results: Electrophysiological and Calcium Recordings in Retinal Wholemount
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

一旦从主体去除，神经元组织大大受环境条件的影响，从而导致组织的最终降解后6 - 8小时。采用了独特的培养方法，该方法密切监测和调节组织的胞外环境中，组织的生存力可以显著延长> 24小时。

Tags

ElectrophysiologyCalcium ImagingBrain Slice PreparationRetinal Whole MountIncubation SystemCarbogen FlowPH ControlTemperature ControlVibratomePapainEDTADNaseOvomuccoidBSAEarle’s BSSACSF

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