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Biochemistry

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JoVE Journal Biochemistry
Forbedret Prøve Multiplexing af væv under anvendelse kombinerede Precursor isotopmærkning og isobar Tagging (cPILOT)
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Forbedret Prøve Multiplexing af væv under anvendelse kombinerede Precursor isotopmærkning og isobar Tagging (cPILOT)

Article DOI: 10.3791/55406-v • 09:06 min • May 1st, 2017
May 1st, 2017 •
1, 1, 2, 3, 1
1Department of Chemistry, University of Pittsburgh, 2SGS North America Inc., 3Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science, Janssen Research and Development

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Kombineret precursor isotopmærkning og isobar tagging (cPILOT) er en kvantitativ proteomics strategi, der forbedrer prøve multiplexing kapaciteter isobare tags. Denne protokol beskriver anvendelsen af ​​cPILOT til væv fra et Alzheimers sygdom-musemodel og vildtypekontroller.

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Biokemi kvantitative proteomics cPILOT TMT isotopmærkning og isobar tagging multiplexing væv
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