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Forbedret Sample Multiplexing av vev ved hjelp Kombinert Precursor Isotop Merking og isobariske Tagging (cPILOT)
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Chapters
- 00:05Title
- 01:14Protein Extraction and Generation of Peptides for Chemical-tagging
- 02:05Sample Digestion
- 03:16Dimethylation Labeling (N-termini)
- 05:13Isobaric Tagging (Lys residues)
- 06:12Liquid Chromatography – Tandem Mass Spectrometry (LC-MS/MS) and MS3
- 07:02Results: cPILOT Can Be Used to Analyze Tissues from an Alzheimer’s Disease Mouse Model and Wild-type Controls
- 08:23Conclusion
Kombinert forløper isotopisk merking og isobarisk merking (cPILOT) er en kvantitativ proteomforskning strategi som øker samplings multipleksing av egenskapene til isobariske koder. Denne protokollen beskriver anvendelsen av cPILOT til vev fra en Alzheimers sykdom musemodellen og villtype-kontroller.
Tags
Enhanced Sample MultiplexingCombined Precursor Isotopic LabelingIsobaric TaggingCPILOTAlzheimer’s DiseaseMouse ModelProtein ChangesAgingNeurodegenerative DiseaseCancerPathogenesisDiagnosisPrognosisTherapeutic TargetsAcetylationProtein NitrationBrainHeartLiverBCA AssayProtein ConcentrationDithiothreitolIodoacetamideL-cysteineTrypsin Digestion
