JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
使用微流体装置来衡量寿命和细胞表型单芽殖酵母细胞
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells
Please note that all translations are automatically generated.
Click here for the English version.
使用微流体装置来衡量寿命和细胞表型单芽殖酵母细胞
DOI:
10.3791/55412-v
•
09:18 min
•
March 30, 2017
•
Ke Zou
2
,
Diana S. Ren
,
Qi Ou-yang
3
,
Hao Li
,
Jiashun Zheng
1
The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics
,
Peking University
,
2
Department of Biochemistry and Biophysics
,
University of California, San Francisco
,
3
Peking-Tsinghua Center for Life Sciences at Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies
,
Peking University
Chapters
00:05
Title
01:22
Microfluidic Device Molding
03:04
Microfluidic Device Preparation and Assembly
04:56
Microfluidic Tube Preparation
06:12
Loading of a Yeast Sample
07:28
Results: Fresh Mother Cells Live Longer than Cells with Unknown History
08:27
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本文介绍用于生产微流体芯片和微流体实验的设置来测量的寿命和单个酵母细胞的细胞表型进行了优化的协议。
Tags
Microfluidic Devices
Lifespan
Cellular Phenotypes
Single Budding Yeast Cells
Yeast Aging
Cell Tracking
Molecular Markers
Secondary Responses
Cell Lineage
PDMS
Silicon Wafer Mold
Device Fabrication
Cell Loading
Microscope Setup
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
在芽殖酵母中的自我复制寿命测量
RNAi筛选识别胚后的表型
C。线虫</em
寿命测量<em>果蝇</em
获取荧光时间推移电影的芽殖酵母和分析单细胞动力学移植物
在单酵母细胞MAPK诱导表达的时空定量
在大规模的基因敲除<em> C。线虫</em>使用双链RNA料库产生失去功能的鲁棒表型
芽殖酵母蛋白质的提取和纯化功能研究,交互和翻译后修饰
pHluorin的申请在芽殖酵母细胞内吞的定量,动力学和高通量分析
在裂殖酵母中Cytokinetic活动的时空分析
高分辨率成像和芽殖酵母个人星体微管动力学分析
Read Article