JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
シングル出芽酵母細胞での寿命と細胞表現型を測定するためのマイクロ流体デバイスを使用しました
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells
Please note that all translations are automatically generated.
Click here for the English version.
シングル出芽酵母細胞での寿命と細胞表現型を測定するためのマイクロ流体デバイスを使用しました
DOI:
10.3791/55412-v
•
09:18 min
•
March 30, 2017
•
Ke Zou
2
,
Diana S. Ren
,
Qi Ou-yang
3
,
Hao Li
,
Jiashun Zheng
1
The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics
,
Peking University
,
2
Department of Biochemistry and Biophysics
,
University of California, San Francisco
,
3
Peking-Tsinghua Center for Life Sciences at Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies
,
Peking University
Chapters
00:05
Title
01:22
Microfluidic Device Molding
03:04
Microfluidic Device Preparation and Assembly
04:56
Microfluidic Tube Preparation
06:12
Loading of a Yeast Sample
07:28
Results: Fresh Mother Cells Live Longer than Cells with Unknown History
08:27
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
この記事では、マイクロ流体チップの生産と寿命と、単一の酵母細胞の細胞表現型を測定するためのマイクロ流体実験のセットアップのために最適化されたプロトコルを提示します。
Tags
Microfluidic Devices
Lifespan
Cellular Phenotypes
Single Budding Yeast Cells
Yeast Aging
Cell Tracking
Molecular Markers
Secondary Responses
Cell Lineage
PDMS
Silicon Wafer Mold
Device Fabrication
Cell Loading
Microscope Setup
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
出芽酵母の複製寿命の測定
の後胚表現型を識別するためのRNAiスクリーニング
C.エレガンス</em
における寿命の測定<em>キイロショウジョウバエ</em
出芽酵母の蛍光タイムラプス作品を取得し、移植片を使用したシングルセルダイナミクスの解析
シングル酵母細胞中のMAPK誘導発現の時間的な定量
大規模な遺伝子ノックダウン中<em> C.虫</em>堅牢な機能喪失表現型を生成するためのdsRNA摂食ライブラリの使用
機能の研究、相互作用、および翻訳後修飾のため出芽酵母タンパク質の抽出と精製
出芽酵母におけるエンドサイトーシスの定量的、動力学的およびハイスループット分析のためのpHluorinの応用
分裂酵母における細胞質分裂のイベントの時空間解析
出芽酵母における高解像度画像と個人アストラル微小管動態の解析
Read Article