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JoVE Journal
Genetics

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用荧光PCR毛细管电泳技术在基因型CRISPR / Cas9介导的敲除突变体的高通量形式
 
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用荧光PCR毛细管电泳技术在基因型CRISPR / Cas9介导的敲除突变体的高通量形式

Article DOI: 10.3791/55586-v 08:25 min April 8th, 2017
April 8th, 2017

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

这里所描述的基因分型技术,荧光聚合酶链式反应(PCR),以毛细管凝胶电泳,其耦合,允许核酸酶介导敲除克隆的高通量基因分型。它规避所面临的其他基因分型技术的限制,并且比测序方法更具成本效益。

Tags

遗传学,第122,基因组编辑,基因敲除,插入/缺失突变,高通量筛选,直接裂解,复用的
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