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基于数字PCR的沙门氏菌健康高通量分析竞争指数
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Genetics
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JoVE Journal Genetics
Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella
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基于数字PCR的沙门氏菌健康高通量分析竞争指数

DOI:
10.3791/59630-v

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07:11 min

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May 13, 2019

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,
1Department of Medical Microbiology and Immunology,Creighton University School of Medicine

Chapters

  • 00:04Title
  • 00:43Validate the Sensitivity and Specificity of Each Primer-probe Set for Each Genomic Barcode Using Digital PCR
  • 03:09Analyze Digital PCR Data and Calculate Absolute Copy Numbers
  • 04:52Results: Representative Digital PCR Results of Diluted AA-barcoded gDNA
  • 06:28Conclusion

Summary

Automatic Translation

Automatic Translation

这种基于分子的确定细菌适应性的方法,使用通过数字PCR量化的独特基因组DNA条形码,有助于精确、准确地检测微生物。该协议描述了计算沙门氏菌菌株的竞争指数;然而,该技术很容易适应需要绝对定量任何基因可塑性有机体的协议。

Tags

Digital PCRCompetitive IndexHigh-throughput AnalysisFitnessSalmonellaQuantificationMixed PopulationMolecular-based TechniquesGenetic MarkersBacterial ChromosomesGenomic DNADilution SeriesDigital PCR SupermixProbe-based ChemistryNo Template ControlsNegative ControlsPositive ControlsDroplet GeneratorThermal CyclerData Analysis Software

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