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Ekstraktion og oprensning af polyphenoler fra frysetørret bærpulver til behandling af vaskulære glatte muskelceller<em> In Vitro</em
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
DOI:

12:00 min

July 05, 2017

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Chapters

  • 00:05Title
  • 01:08Preparation of Crude Blackberry Extracts
  • 03:31Preparation of Polyphenol Extracts from Crude Berry Extracts
  • 05:13Treatment of Vascular Smooth Muscle Cells with Berry Extracts-purified Polyphenols
  • 09:08Results: Polyphenol Composition and Phosphorylation Reduction by Crude and Purified Berry Extracts
  • 11:05Conclusion

Summary

Automatic Translation

Dette arbejde beskriver en trin-for-trin metode til fremstilling af polyphenol-rige ekstrakter fra frysetørret bærpulver. Derudover giver den en grundig beskrivelse af, hvordan man bruger disse polyphenolrige ekstrakter i cellekultur i nærvær af peptidhormon angiotensin II (Ang II) ved hjælp af vaskulære glatte muskelceller (VSMC'er).

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