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A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
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JoVE Journal Chemistry
A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

DOI:
10.3791/56445-v

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13:35 min

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March 01, 2018

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, , , ,
1School of Public Health of Southeast University,Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, 2Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering,Southeast University, 3School of Public Health,Tianjin Medical University, 4British Columbia Academy,Nanjing Foreign Language School

Chapters

  • 00:04Title
  • 01:27Preparation of the Columns and Solutions Needed for the Extraction and Determination of Catecholamines
  • 04:44Preparation of Real Urine Samples and Mobile Phase
  • 06:25PFSPE Extraction and HPLC Analysis
  • 09:58Results: Identification and Quantification of Extracted Catecholamines and their Metabolites in Urine
  • 11:43Conclusion

Summary

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We present a convenient solid-phase extraction coupled to high-pressure liquid chromatography (HPLC) with electrochemical detection (ECD) for simultaneous determination of three monoamine neurotransmitters and two of their metabolites in infants' urine. We also identify the metabolite MHPG as a potential biomarker for the early diagnosis of brain damage for infants.

Tags

Keywords: High-pressure Liquid ChromatographyCatecholamine NeurotransmittersMetabolitesSolid-phase ExtractionElectrochemical DetectionMonoamine NeurotransmittersInfant’s UrineDPBA SolutionNorepinephrineEpinephrineDopamineMHPGDOPACDHBAHydrochloric AcidAnalyte Stocks

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