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从冷冻干燥的浆果粉中提取和纯化多酚用于治疗血管平滑肌细胞<em>体外</em
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Chemistry
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JoVE Journal Chemistry
Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
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从冷冻干燥的浆果粉中提取和纯化多酚用于治疗血管平滑肌细胞<em>体外</em

DOI:
10.3791/55605-v

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12:00 min

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July 05, 2017

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, , , , ,
1Department of Dietetics and Nutrition,University of Arkansas for Medical Sciences, 2Department of Nutrition, Food and Exercise Sciences,Florida State University, 3Center for Advancing Exercise and Nutrition Research on Aging (CAENRA),Florida State University

Chapters

  • 00:05Title
  • 01:08Preparation of Crude Blackberry Extracts
  • 03:31Preparation of Polyphenol Extracts from Crude Berry Extracts
  • 05:13Treatment of Vascular Smooth Muscle Cells with Berry Extracts-purified Polyphenols
  • 09:08Results: Polyphenol Composition and Phosphorylation Reduction by Crude and Purified Berry Extracts
  • 11:05Conclusion

Summary

Automatic Translation

Automatic Translation

本工作详细介绍了从冷冻干燥的浆果粉中制备富含多酚的提取物的逐步方法。此外，它提供了在使用血管平滑肌细胞（VSMC）的肽激素血管紧张素II（Ang II）存在下如何在细胞培养物中使用这些富含多酚的提取物的详细描述。

Tags

Polyphenol ExtractionFreeze-dried Berry PowderUltrasonic-assisted ExtractionEthanol-based ExtractionChloroform PurificationEthyl Acetate FractionationVascular Smooth Muscle CellsIn Vitro Treatment

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