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高分辨率成像和芽殖酵母个人星体微管动力学分析

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Biology

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JoVE Journal Biology

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

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高分辨率成像和芽殖酵母个人星体微管动力学分析

DOI:

10.3791/55610-v

•

10:23 min

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April 20, 2017

•

Colby P. Fees, Cassi Estrem, Jeffrey K. Moore

¹Department of Cell and Developmental Biology,University of Colorado School of Medicine

Chapters

00:05Title
00:38Growing Liquid Yeast Culture
01:26Preparing Flow Chamber Slides
03:36Loading Yeast Cells into Prepared Flow Chamber Slides
05:03Image Acquisition
06:30Image Analysis
08:15Results: Astral Microtubule Dynamics in Altered in tub2-430Δ Mutant Cells
09:32Conclusion

Summary

Automatic Translation

出芽酵母是研究体内微管动力学由于其强大的遗传学和其微管骨架的简单性的有利的模型。以下方案描述了如何变换和培养的酵母细胞，获得共焦显微镜图像，和定量分析在活酵母细胞的微管动力学。

Tags

Microtubule Dynamics Yeast Cells GFP-labeled Tubulin Flow Chamber Slides Paraffin Film Time-lapse Imaging Microscopy Cell Biology Cytoskeleton

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