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啮齿动物脑 Microinjections 的个性化针

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Personalized Needles for Microinjections in the Rodent Brain

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啮齿动物脑 Microinjections 的个性化针

DOI:

10.3791/55751-v

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10:06 min

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January 24, 2018

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Giovanna Paolone*¹, Chiara Falcicchia*¹, Gianluca Verlengia*¹, Mario Barbieri, Anna Binaschi, Federico Paliotto, Beatrice Paradiso, Marie Soukupova, Silvia Zucchini², Michele Simonato²

¹Department of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences,University of Ferrara, ²Laboratory for the Technologies for Advanced Therapies (LTTA),University of Ferrara

Chapters

00:05Title
00:56Preparation of Quartz Needles
03:18Microinjection
08:03Results: Coronal Sections Showing Less Damage Using the Quartz Needle
08:56Conclusion

Summary

Automatic Translation

我们在这里描述了一种使用石英针的啮齿动物脑内注射的协议。这些针头不会产生可检测的组织损伤, 并确保即使在深部区域也能可靠地提供。此外, 他们可以适应研究需要的个性化设计, 可以重新使用。

Tags

Personalized Quartz Needles Microinjection Rodent Brain Neuroscience Neurodegenerative Diseases Psychiatric Diseases Gene Therapy Cell Therapy Cost-effective Reusable Laser Puller Micro Dissector Void Pump Flow Rate Calibration

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