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1Curriculum in Toxicology,University of North Carolina at Chapel Hill, 2National Toxicology Program Laboratory (NTPL), DNTP,National Institute of Environmental Health Sciences, 3Cellular and Molecular Pathology Branch, DNTP,National Institute of Environmental Health Sciences, 4Charles River Laboratories Inc.
Chapters
- 00:05Title
- 01:08Removal of Mammary Whole Mount from a Glass Slide
- 02:30Tissue Processing
- 03:52Embedding the Processed Mammary Tissue
- 05:10Sectioning the Paraffin-embedded Mammary Tissue on the Microtome and Automated H and E Staining
- 06:51Results: Lesion Identification in Whole Mount Mammary Gland Sections
- 08:23Conclusion
Desenvolvemos um método para remover, processar, cortar e manchar com sucesso, para avaliação histopatológica, tecido mamário que inicialmente havia sido fixado em slides como montagens inteiras. Este método pode promover a coleta e avaliação de montantes inteiros de glândula mamária em estudos de diretrizes de testes reprodutivos e de desenvolvimento.
