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同时测量 HDAC1 和 HDAC6 活动在 HeLa 细胞使用 UHPLC 女士
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS
DOI:

09:20 min

August 10, 2017

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Chapters

  • 00:05Title
  • 01:20Cell Culture
  • 03:00Cell Treatment with Cell Compounds and HDAC Substrates, Cell Lysis, and Sample Preparation for UHPLC-ESI-MS/MS
  • 05:14UHPLC/MS-MS Analysis and Data Analysis
  • 06:14Results: Deacetylation of Specific Substrates by Endogenous HDACs and Potency of Standard HDAC Inhibitors
  • 08:18Conclusion

Summary

Automatic Translation

本方法用于确定亚型特异性抑制剂组蛋白去乙酰化酶 (HDAC) 在 HeLa 细胞中由多个基板 UHPLC 气相色谱-质谱分析。这是一种抗体免费的方法,开发以反映生活的 HDAC1 和 HDAC6 活性与单一异构体无细胞检测的细胞环境。

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