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Eksperimentelle modeller for å studere nevrobeskyttelsen av sur postkonditionering mot cerebral iskemi
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Experimental Models to Study the Neuroprotection of Acidic Postconditioning Against Cerebral Ischemia
DOI:

10:13 min

July 31, 2017

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Chapters

  • 00:05Title
  • 00:57Cortical Slice Preparation
  • 02:43Oxygen-glucose Deprivation and Acidic Postconditioning
  • 04:03Determination of Slice Viability
  • 05:04Middle Cerebral Artery Occlusion
  • 07:22Reperfusion and Acidic Postconditioning
  • 08:16Results: Acidic Preconditioning Protects Against Reperfusion Injury
  • 09:32Conclusion

Summary

Automatic Translation

Syrenettkondisjonering beskytter mot cerebral iskemi. Her presenterer vi to modeller for å utføre APC. De er oppnådd henholdsvis ved overføring corticostriatal skiver til sur buffer etter oksygen-glukosemangel in vitro, og ved inhalering 20% CO2 etter tilstopping av midtre cerebralarterie in vivo.

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