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Journal
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Biochemistry
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用耐热配体测量生物分子 DSC 剖面快速表征折叠和结合的相互作用
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Biochemistry
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JoVE Journal
Biochemistry
Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
Please note that all translations are automatically generated.
Click here for the English version.
用耐热配体测量生物分子 DSC 剖面快速表征折叠和结合的相互作用
DOI:
10.3791/55959-v
•
09:15 min
•
November 21, 2017
•
Robert W. Harkness V
,
Philip E. Johnson
,
Anthony K. Mittermaier
1
Department of Chemistry
,
McGill University
,
2
Department of Chemistry
,
York University
Chapters
00:05
Title
00:44
Sample Preparation
02:11
DSC Preparation
05:32
Collecting Thermolabile Ligand DSC Datasets
07:39
Results: Rapid Characterization of Biomolecular Folding and Binding Interactions with Thermolabile Ligands by DSC
08:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一个协议, 快速表征生物分子折叠和约束力的相互作用与耐热配体使用差分扫描量热法。
Tags
Biomolecular Folding
Binding Interactions
Differential Scanning Calorimetry (DSC)
Thermolabile Ligand
Thermodynamic Parameters
Biocalorimetry
Drug Design
Ligand Titration
Dialysis
Buffer Matching
Biomolecule Concentration
Degassing
DSC Setup
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