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用耐热配体测量生物分子 DSC 剖面快速表征折叠和结合的相互作用
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
DOI:

09:15 min

November 21, 2017

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Chapters

  • 00:05Title
  • 00:44Sample Preparation
  • 02:11DSC Preparation
  • 05:32Collecting Thermolabile Ligand DSC Datasets
  • 07:39Results: Rapid Characterization of Biomolecular Folding and Binding Interactions with Thermolabile Ligands by DSC
  • 08:21Conclusion

Summary

Automatic Translation

我们提出了一个协议, 快速表征生物分子折叠和约束力的相互作用与耐热配体使用差分扫描量热法。

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