Journal
/
Biochemistry
/
Measuring Protein Binding to F-actin by Co-sedimentation
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
Measuring Protein Binding to F-actin by Co-sedimentation

Measuring Protein Binding to F-actin by Co-sedimentation

DOI:
10.3791/55613-v

•

06:17 min

•

May 18, 2017

•

, ,
1Department of Cell Biology,University of Pittsburgh School of Medicine, 2Department of Biology,University of North Carolina at Chapel Hill

Chapters

  • 00:05Title
  • 00:33Prepare the Test Protein and F-actin for the Assay
  • 02:02Pelleting Assay – Quantification
  • 04:33Results: αE-catenin Homodimer Binds F-actin
  • 05:16Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a method to test the ability of a protein to co-sediment with filamentous actin (F-actin) and, if binding is observed, to measure the affinity of the interaction.

Tags

Keywords: Protein BindingF-actinCo-sedimentationActin PolymerizationUltracentrifugationProtein ConcentrationBinding AssayAffinity Measurement

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55613?article_type=v

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
check_url/55613?article_type=v

In Vitro Polymerization of F-actin on Early Endosomes
check_url/55613?article_type=v

Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
check_url/55613?article_type=v

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
check_url/55613?article_type=v

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
check_url/55613?article_type=v

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
check_url/55613?article_type=v

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes
check_url/55613?article_type=v

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
check_url/55613?article_type=v

Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments
check_url/55613?article_type=v

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Read Article