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Neuroscience
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自动图像引导贴片夹在脑切片神经元研究中的应用
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices
Please note that all translations are automatically generated.
Click here for the English version.
自动图像引导贴片夹在脑切片神经元研究中的应用
DOI:
10.3791/56010-v
•
09:05 min
•
July 31, 2017
•
Qiuyu Wu
2
,
Alexander A. Chubykin
2
1
Department of Biological Sciences
,
Purdue University
,
2
Purdue Institute for Integrative Neuroscience
,
Purdue University
Chapters
00:05
Title
01:16
Run Autopatcher IG
02:48
Automatic Patch Clamp Procedure
07:03
Results: Conducting an Automated Drug Delivery Experiment
08:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了如何使用最近为标准
体外
电生理设备开发的系统进行自动图像引导的膜片钳实验。
Tags
Automated Image-guided Patch Clamp
Neurons
Brain Slices
Computer Vision
Fluorescent Cells
Patch Clamp Electrophysiology
High Throughput
Neurological Disorders
Pharmacological Screening
Neuronal Preparations
Brain Slice
Slice Culture
Dissociated Neurons
Calibration
Manipulator
Microscope
Amplifier
Autopatcher IG
Internal Solution
Pipette Tip
Microscope Stage
Coordinate System
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