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Chapters
- 00:05Title
- 00:41Day 1: Primary Immunostaining of Mouse Muscle Sections
- 01:57Day 2: Secondary Immunostaining of Mouse Muscle Sections
- 03:24Collecting Muscle Images and their Analysis with the Fiber Quantification Macro
- 06:23Results: Representative Immunostaining and Morphometric Analysis
- 07:50Conclusion
Immunohistochemical farging av myosin tunge kjeden isoformene har dukket opp som den state-of-the-art diskriminator skjelettlidelser muskel fiber-type (dvs.type I-, skriver IIA, Skriv inn IIX, skriver IIB). Her presenterer vi en fargeprotokoll sammen med en roman halvautomatisk algoritme som muliggjør rask vurdering av fiber-type og fiber morfologi.
