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Bestemmelse af sialinsyrer i lever- og mælkeprøver af vildtype og<em> CMAH</em> Knock-out Mus.
JoVE Journal
Biology
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JoVE Journal Biology
Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.
DOI:

08:04 min

July 14, 2017

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Chapters

  • 00:05Title
  • 00:55Isolation of Sialic Acids from Milk and from Liver Tissue
  • 03:26Preparation of a Mixed Sialic Acid Standard
  • 04:26Fluorescence Derivatization of Sialic Acids
  • 05:18HPLC Analysis of Sialic Acid Derivatives
  • 06:15Results: Quantification of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice
  • 07:05Conclusion

Summary

Automatic Translation

Vi beskriver en HPLC-baseret metode til bestemmelse af N-acetylneuraminsyre og N-glycolylenuraminsyre i muselever og mælk.

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