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Bepaling van Sialic Acids in Lever en Melk Monsters van Wild-type en<em> CMAH</em> Knock-out Muizen.
JoVE Journal
Biology
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JoVE Journal Biology
Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.
DOI:

08:04 min

July 14, 2017

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Chapters

  • 00:05Title
  • 00:55Isolation of Sialic Acids from Milk and from Liver Tissue
  • 03:26Preparation of a Mixed Sialic Acid Standard
  • 04:26Fluorescence Derivatization of Sialic Acids
  • 05:18HPLC Analysis of Sialic Acid Derivatives
  • 06:15Results: Quantification of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice
  • 07:05Conclusion

Summary

Automatic Translation

We beschrijven een HPLC-gebaseerde methode voor de bepaling van N-acetylneuraminezuur en N-glycolylenuraminezuur in muizenlever en melk.

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