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Chapters
- 00:05Title
- 01:04Generating Embryos Through Natural Mating and Staging Embryos
- 02:22Whole Embryo Dissociation, RNA Extraction and Purify High-quality RNA
- 04:55Sorting Differentially Expressed Genes
- 07:00Determine Enriched Pathways
- 07:57Generation of Pathway Networks and Determination of Enriched GO Terms
- 09:12Results: RNAseq-based Zebrafish Genome Expression Data for Alstrom and Bardet Biedl Syndromes
- 10:47Conclusion
Ce protocole présente une approche pour l’analyse du transcriptome ensemble des embryons de poisson-zèbre, larves, ou trier des cellules. Nous incluons l’isolement de l’ARN, la voie analyse des données RNASeq et qRT-PCR-based validation des modifications de l’expression génique.
