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Genetics

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Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow
 

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

Article doi: 10.3791/55812
June 14th, 2017

Summary June 14th, 2017

This manuscript describes the experimental procedure and software analysis for a bidirectional integration site assay that can simultaneously analyze upstream and downstream vector-host junction DNA. Bidirectional PCR products can be used for any downstream sequencing platform. The resulting data are useful for a high-throughput, quantitative comparison of integrated DNA targets.

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