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骨骼肌损伤诱导衰老和体内重编程的评价
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Developmental Biology
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JoVE Journal Developmental Biology
Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle
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骨骼肌损伤诱导衰老和体内重编程的评价

DOI:
10.3791/56201-v

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09:14 min

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October 26, 2017

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, ,
1Cellular Plasticity & Disease Modelling, Department of Developmental & Stem Cell Biology, CNRS UMR 3738,Institut Pasteur

Chapters

  • 00:05Title
  • 01:00SA-β-Gal Staining of Frozen TA Muscle Section
  • 02:17Immunohistochemistry Using Anti-Nanog Antibody
  • 04:38Analysis and Quantification
  • 07:30Results: Evaluation of In Vivo Reprogramming After Muscle Injury
  • 08:16Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出了一个详细的协议, 以检测两个衰老和多能干细胞的骨骼肌肉损伤时, 诱导在体内重新编程。该方法适用于评价细胞衰老在组织再生过程中的作用和重新编程体内。

Tags

SenescenceReprogrammingSkeletal MuscleRegenerative MedicineX-gal StainingImmunohistochemistryNanogParaformaldehydeGlutaraldehyde

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