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骨骼肌损伤诱导衰老和体内重编程的评价
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle
DOI:

09:14 min

October 26, 2017

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Chapters

  • 00:05Title
  • 01:00SA-β-Gal Staining of Frozen TA Muscle Section
  • 02:17Immunohistochemistry Using Anti-Nanog Antibody
  • 04:38Analysis and Quantification
  • 07:30Results: Evaluation of In Vivo Reprogramming After Muscle Injury
  • 08:16Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个详细的协议, 以检测两个衰老和多能干细胞的骨骼肌肉损伤时, 诱导在体内重新编程。该方法适用于评价细胞衰老在组织再生过程中的作用和重新编程体内

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