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一种在透射电镜中获取微生物连续超薄切片的方法
JoVE Journal
Engineering
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JoVE Journal
Engineering
A Method for Obtaining Serial Ultrathin Sections of Microorganisms in Transmission Electron Microscopy
Please note that all translations are automatically generated.
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一种在透射电镜中获取微生物连续超薄切片的方法
DOI:
10.3791/56235-v
•
09:46 min
•
January 17, 2018
•
Masashi Yamaguchi
,
Hiroji Chibana
1
Medical Mycology Research Center
,
Chiba University
Chapters
00:05
Title
00:45
Preparation of Support Film
01:53
Trimming the Specimen Block
03:31
Preparing the Specimen
04:59
Making Serial Sections
06:18
Picking up Serial Sections
07:23
Staining and Observation of Serial Sections
08:14
Results: Serial Ultrathin Sections of Microorganisms
09:18
Conclusion
Summary
Automatic Translation
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Automatic Translation
本研究为在透射电子显微镜下获取无昂贵设备的微生物的连续超薄切片提供了可靠、简便的程序。
Tags
Ultrathin Sections
Transmission Electron Microscopy
Serial Sectioning
Formvar Film
Specimen Block Trimming
Diamond Knife
Microtome
Cell Biology
Three-dimensional Structural Information
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