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1School of Fisheries, Aquaculture and Aquatic Sciences,Auburn University, 2Department of Animal Wealth Development, Faculty of Veterinary Medicine,Suez Canal University, 3Anatomy and Embryology Department, Faculty of Veterinary Medicine,Cairo University, 4Department of Molecular Physiology and Biophysics,Vanderbilt University, 5Life Science Institute,University of Michigan
Chapters
- 00:00Title
- 01:14Brood Stock Selection and Spawning
- 04:16Sperm Preparation
- 05:33Egg Collection and Fertilization
- 08:07Needle Pulling and Loading
- 10:20Microinjection of Catfish Embryos
- 12:34Results
- 15:24Conclusion
En enkel og effektiv mikroinjektion protokol til gen redigering i kanal havkat embryoner ved hjælp af CRISPR/Cas9 system er præsenteret. I denne protokol, guide RNA'er og Cas9 protein var microinjected i blommesækken af én-celle embryoner. Denne protokol er blevet valideret af udskære to kanal havkat immun-relaterede gener.
