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Journal
/
Medicine
/
体外
和
体内
检测人类细胞中的 Mitophagy,
线虫
和小鼠
JoVE Journal
Medicine
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JoVE Journal
Medicine
In Vitro
and
In Vivo
Detection of Mitophagy in Human Cells,
C. Elegans
, and Mice
Please note that all translations are automatically generated.
Click here for the English version.
体外
和
体内
检测人类细胞中的 Mitophagy,
线虫
和小鼠
DOI:
10.3791/56301-v
•
08:40 min
•
November 22, 2017
•
Evandro F. Fang
6
,
Konstantinos Palikaras
,
Nuo Sun
,
Elayne M. Fivenson
,
Ryan D. Spangler
,
Jesse S. Kerr
,
Stephanie A. Cordonnier
,
Yujun Hou
,
Eszter Dombi
,
Henok Kassahun
,
Nektarios Tavernarakis
7
,
Joanna Poulton
,
Hilde Nilsen
,
Vilhelm A. Bohr
8
1
Laboratory of Molecular Gerontology, National Institute on Aging
,
National Institutes of Health
,
2
Institute of Molecular Biology and Biotechnology
,
Foundation for Research and Technology – Hellas
,
3
Center for Molecular Medicine, National Heart Lung and Blood Institute
,
National Institutes of Health
,
4
Laboratory of Neurosciences, National Institute on Aging
,
National Institutes of Health
,
5
Nuffield Department of Obstetrics and Gynaecology
,
University of Oxford
,
6
Department of Clinical Molecular Biology
,
University of Oslo and Akershus University Hospital
,
7
Department of Basic Sciences, Faculty of Medicine
,
University of Crete
,
8
Danish Center for Healthy Aging
,
University of Copenhagen
Chapters
00:05
Title
01:30
Detection of Mitophagy in Human Cells using mt-Keima Plasmid
03:12
Assessing Mitophagy using Co-localization Between DCT-1 and LGG-1 in
C. elegans
05:36
Detection of Mitophagy in Mice
06:19
Results: Evaluation of Mitophagy in Human Cells,
C. elegans
, and Mice
07:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
Mitophagy, 清除受损线粒体的过程, 是线粒体稳态和健康维护所必需的。本文介绍了人类细胞、
线虫线虫
和小鼠的一些最新的 mitophagy 检测方法。
Tags
Mitophagy
In Vitro
In Vivo
Human Cells
C. Elegans
Mice
Neuro-degeneration
Metabolic Diseases
Aging
Mito-Keima
Confocal Microscopy
Transfection
Cell Culture
Article
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