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乳腺癌细胞系外周血单核细胞破的人前临床模型的建立
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Cancer Research
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JoVE Journal Cancer Research
Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines
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乳腺癌细胞系外周血单核细胞破的人前临床模型的建立

DOI:
10.3791/56311-v

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06:00 min

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September 13, 2017

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, , , , , , , ,
1Osteoncology and Rare Tumors Center, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST),IRCCS

Chapters

  • 00:05Title
  • 01:17Osteoclast Differentiation and Cancer Cell Co-culture
  • 04:06Results: Representative Effects of Everolimus on Osteoclastogenesis Treatment
  • 04:51Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了一个体外的发展, 从外周血单核细胞中培养的乳癌细胞系破人前模型, 模拟癌细胞-破骨细胞的相互作用。该模型可用于进一步了解骨转移形成和改善治疗方案。

Tags

OsteoclastogenesisPeripheral Blood MonocytesBreast Cancer Cell LinesBone MetastasisPreclinical ModelPBMCMCSFTRAP Staining

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