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JoVE Journal Cancer Research
Påvisning af mitokondrier membran potentiale til at studere CLIC4 Knockdown-induceret HN4 celle apoptose In Vitro
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Påvisning af mitokondrier membran potentiale til at studere CLIC4 Knockdown-induceret HN4 celle apoptose In Vitro

Article DOI: 10.3791/56317-v • 13:20 min • July 17th, 2018
July 17th, 2018 •
1, 2, 2, 2, 2, 2
1School of Basic Medical Sciences, Anhui Medical University, 2Department of Orthopedics, Anhui Provincial Hospital, Anhui Medical University

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Her præsenterer vi en detaljeret protokol om anvendelse af rhodaminfilteret 123 til at identificere den mitokondrielle membran potentiale (MMP) og studere CLIC4 knockdown-induceret HN4 celle apoptose in vitro. Under fælles fluorescens mikroskop og konfokal laser scanning fluorescens mikroskop, blev real-time ændringen af MMP indspillet.

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Kræftforskning sag 137 mitokondrielle membran potentiale rodamin 123 Konfokal laser scanning fluorescens mikroskop chlorid intracellulære kanal 4 apoptose hoved og hals planocellulære karcinom
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