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MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening

DOI:
10.3791/56326-v

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08:14 min

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October 26, 2017

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, , , , , , , ,
1Department of Experimental Oncology and Molecular Medicine,Fondazione IRCCS Istituto Nazionale dei Tumori, 2Unit of Thoracic Surgery,Fondazione IRCCS Istituto Nazionale dei Tumori

Chapters

  • 00:05Title
  • 01:12Plasma Sample Collection and RNA Extraction
  • 03:20Taq-based Reverse Transcription and Pre-amplification
  • 04:34RT-qPCR Reaction
  • 05:46Results: Quality Control of Plasma Samples and RT-qPCR Performance Evaluation
  • 07:15Conclusion

Summary

Automatic Translation

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Here, we present the detailed protocol adopted in the BioMILD screening trial to perform the circulating microRNA signature classifier test for early lung cancer detection.

Tags

Keywords: Liquid BiopsyPlasma MiRNALung Cancer ScreeningMicroRNA SignatureEarly DetectionTumor MicroenvironmentRNA IsolationQPCRMolecular Analysis

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