Journal
/
Cancer Research
/
Betinget Knockdown af genekspression i kræft cellelinjer til at studere ansættelse af monocytter/makrofager at Tumor mikromiljø
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Betinget Knockdown af genekspression i kræft cellelinjer til at studere ansættelse af monocytter/makrofager at Tumor mikromiljø

DOI:
10.3791/56333-v

•

10:59 min

•

November 23, 2017

•

,
1Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Pediatrics,University of Southern California, 2The Saban Research Institute of Children’s Hospital Los Angeles, 3Department of Biochemistry and Molecular Medicine,University of Southern California

Chapters

  • 00:05Title
  • 01:09Generation of Lentivirus Particles
  • 02:34Generation of Stably Transfected Lines
  • 04:55Isolation of Monocytes from Human Blood
  • 08:00Boyden Chamber Migration Assay
  • 09:25Results: PAI-1 Promotes Macrophage Migration
  • 10:24Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol fungerer som en ordning for oprettelse af en funktionel Tet-ON system i kræft cellelinjer og dens efterfølgende anvendelse, navnlig for at studere rollen af tumor celle-afledte proteiner i rekruttering af monocytter/makrofager at tumor mikromiljø.

Tags

Conditional Gene KnockdownCancer Cell LinesMonocyte RecruitmentMacrophage RecruitmentTumor MicroenvironmentLentiviral TransductionHEK-293 CellsHCT-116 CellsMDA-MB-231 CellsMonocyte PurificationPuromycin Selection

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Lunge Tumor celle rekruttering Assay

Det<em> Drosophila</em> Imaginære Disc Tumor Model: Visualisering og Kvantificering af genekspression og tumorindtrængen Brug Genetic Mosaics

Påvisning af mitokondrier membran potentiale til at studere CLIC4 Knockdown-induceret HN4 celle apoptose In Vitro

In Vivo EPR vurdering af pH, pO2, Redox Status, og koncentrationen af fosfat og glutathion i Tumor mikromiljø

Virus levering af CRISPR Guides til Murine prostata for gen ændring

Live-celle Imaging Assays til undersøgelse glioblastom hjerne Tumor stamcelle Migration og Invasion

Oprettelse af cellelinjer overekspression DR3 for at vurdere den apoptotiske reaktion på anti-mitotiske Therapeutics

Analyse af tumor Genekspressions faktorer med CorExplorer-webportalen

Analyse af sidepopulation i faste tumorcellelinjer

Kombineret betinget knockdown og tilpasset sfære dannelse assay at studere en stemness-associerede gene af patient-afledte gastrisk kræft stamceller

Read Article