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Journal / Cancer Research / Betinget Knockdown af genekspression i kræft…
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Cancer Research

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JoVE Journal Cancer Research
Betinget Knockdown af genekspression i kræft cellelinjer til at studere ansættelse af monocytter/makrofager at Tumor mikromiljø
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Betinget Knockdown af genekspression i kræft cellelinjer til at studere ansættelse af monocytter/makrofager at Tumor mikromiljø

Article DOI: 10.3791/56333-v • 10:59 min • November 23rd, 2017
November 23rd, 2017 •
1,2, 1,2,3
1Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Pediatrics, University of Southern California, 2The Saban Research Institute of Children's Hospital Los Angeles, 3Department of Biochemistry and Molecular Medicine, University of Southern California

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Denne protokol fungerer som en ordning for oprettelse af en funktionel Tet-ON system i kræft cellelinjer og dens efterfølgende anvendelse, navnlig for at studere rollen af tumor celle-afledte proteiner i rekruttering af monocytter/makrofager at tumor mikromiljø.

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Kræftforskning sag 129 betinget banke ned celle migration doxycyclin shRNA PAI-1 monocytter kræft cellelinjer
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