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JoVE Journal
Neuroscience

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封闭系统中脑切片长期培养中精确定位和重复间歇成像的改进辊筒法
 
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封闭系统中脑切片长期培养中精确定位和重复间歇成像的改进辊筒法

Article DOI: 10.3791/56436
December 28th, 2017

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Summary December 28th, 2017

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本文介绍了一种改进的滚子管法培养和间歇高分辨率成像啮齿动物脑切片多周, 精确重新定位光刻片。神经元的生存能力和切片形态学保持良好。提供了这种完全封闭的系统使用病毒的细胞类型的特定表达的应用。

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